ABOUT USThe Institute of Human Genetics (IHG) began in 1990 as the Medical Genetics Unit of the University of the Philippines College of Medicine which in 1999 became as one of the service and research oriented institutes of the National Institutes of Health-University of the Philippines Manila (NIH-UP). Since then all services of the Medical Genetics Unit were transferred to the Institute and is now the largest provider of genetic services in the country. |
Microarray Core Laboratory
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The Microarray Core Laboratory hosts two of the country’s microarray facilities and conducts research services on the ethnic population studies, pharmacogenetics, and the genetic basis of commonly prevalent diseases among Filipinos using high-throughput screening and expression profiling of candidate genes. Completed research involved the identification of some of the variants associated with the following diseases among Filipinos: hypertension, coronary artery disease and myocardial infarction, dyslipidemia, diabetes mellitus, systemic lupus erythematosus, asthma, leptospirosis and sepsis. The laboratory is also actively involved in the translation of the results of these researches into diagnostic or prognostication kits which may help aid clinicians in their care for patients with these diseases. |
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What is Microarray? Variations in DNA can result in various conditions or phenotypes. Single nucleotide polymorphisms (or SNPs) are sequences that occur when a nucleotide in the DNA differs between paired chromosomes or biological species. Microarray is a technique used to detect multiple target sequences in an organism’s genome. It can genotype multiple regions in the DNA (deoxyribonucleic acid), can be used to measure expression levels of large number of genes using RNA (ribonucleic acid) simultaneously, and it can also detect changes in DNA methylation.
These applications can be used to: look for biomarkers associated with certain conditions; seek molecular targets for manipulation; and explain molecular processes related to a trait.
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Currently, the Microarray Core Laboratory has on-going programs and projects on the following diseases:
The HiScan™ Reader functions as a high-speed, precision imaging scanner for Illumina sequencing and microarray-based analyses. It is a high-resolution optical imaging instrument that uses red and green lasers to detect fluorescence information on BeadChips.
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PROJECT RECRUITMENT |
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Services offered to research proponents and investigators include:
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WATCH VIDEOS
| Genetic susceptibility to cardiovascular diseases | Genetics of diabetes mellitus | Pharmacogenetic studies on commonly-used cardiovascular drugs |
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Ongoing Programs/Projects: Title: Evaluation of plasma therapy for retarding progression and preventing complications in COVID-19
Publications: Nevado Jr, J. B., & Imasa, M. S. B. (2008). Association of Homocysteine Levels to Traditional Risk Factors in Unstable Angina and Non-ST Elevation Myocardial Infarction: Implications from the FINEST study. Acta Medica Philippina, 42(1). Nevado Jr, J. B., & Imasa, M. S. B. (2008). Homocysteine predicts adverse clinical outcomes in unstable angina and non-ST elevation myocardial infarction: implications from the folate intervention in non-ST elevation myocardial infarction and unstable angina study. Coronary Artery Disease, 19(3), 153-161. Imasa, M. S. B., Gomez, N. T., & Nevado Jr, J. B. (2009). Folic acid-based intervention in non-ST elevation acute coronary syndromes. Asian Cardiovascular and Thoracic Annals, 17(1), 13-21. Cooley, B. C., Nevado, J., Mellad, J., Yang, D., Hilaire, C. S., Negro, A., ... & Boehm, M. (2014). TGF-β signaling mediates endothelial-to-mesenchymal transition (EndMT) during vein graft remodeling. Science Translational Medicine, 6(227), 227ra34-227ra34. Cutiongco-de la Paz, E. M., Ngelangel, C. A., David-Wang, A., Nevado Jr, J. B., Silao, C. L. T., Hernandez-Sebastian, R., ... & Padilla, C. D. (2017). The Prevalence of CYP2D6 Gene Polymorphisms among Filipinos and their use as Biomarkers for Lung Cancer Risk. Acta Medica Philippina, 51(3). Albao, D. S., Cutiongco-de la Paz, E. M., Mercado, M. E., Lirio, A., Mariano, M., Kim, S., ... & Seielstad, M. (2019). Methylation changes in the peripheral blood of Filipinos with type 2 diabetes suggest spurious transcription initiation at TXNIP. Human Molecular Genetics, 28(24), 4208-4218. Reganit PFM, Nevado Jr. JB, Cutiongco-de la Paz EMC, Taquiso JL, Aman AYCL,…, and Sy RG. (2020). BAG6 Variant rs805303 is Nominally Associated with ACEi-induced Cough Among Filipinos. Philippine Journal of Science, Vol. 149 (1): pp. 35-41. Sy RG, Nevado Jr. JB, Llanes EJB, Magno JDA, Ona DID, Aman AYCL,…, and Cutiongco-de la Paz EMC. (2020). The KLOTHO Variant rs36217263 Is Associated With Poor Response to Cardioselective Beta-Blocker Therapy Among Filipinos. Clinical Pharmacology and Therapeutics, Vol. 107(1): pp. 221-226. Poblete, J. M. S., Ballinger, M. N., Bao, S., Alghothani, M., Nevado Jr, J. B., Eubank, T. D., ... & Magalang, U. J. (2020). Macrophage HIF-1α mediates obesity-related adipose tissue dysfunction via interleukin-1 receptor-associated kinase M. American Journal of Physiology-Endocrinology and Metabolism, 318(5), E689-E700. Hilario, A. L., Medina, J. R. C., & Nevado Jr, J. B. (2020). Bioinformatic Analysis of Frequently Reported Somatically Mutated Genes of Triple-negative Breast Cancer and Comparison with Top Mutated Genes from the BRCA-UK Project. Philippine Journal of Science, 149(4), 1243-1252. Santos LG, Nevado JB, Cutiongco-de la Paz EC, Abrahan LL, Aman AYCL,…, and Sy RG. (2021). Variants Near CETP, MTTP and BUD13-ZPR1-APOA5 may be Nominally Associated with Poor Statin Response Among Filipinos. Acta Medica Philippina: Online First. https://actamedicaphilippina.upm.edu.ph/index.php/acta/article/view/2353 Sy RG, Nevado JB, Gonzales EM, Bejarin AP, Aman AYCL,…, and Cutiongco- de la Paz EC. (2021). A Genetic Polymorphism in GCKR may be Associated with Low High-Density Lipoprotein Cholesterol Phenotype among Filipinos: A Case-Control Study. Acta Medica Philippina: Online First. https://actamedicaphilippina.upm.edu.ph/index.php/acta/article/view/2350/. Runcharoen, C., Fukunaga, K., Sensorn, I., Iemwimangsa, N., Klumsathian, S., Tong, H., ... & Chantratita, W. (2021). Prevalence of pharmacogenomic variants in 100 pharmacogenes among Southeast Asian populations under the collaboration of the Southeast Asian Pharmacogenomics Research Network (SEAPharm). Human Genome Variation, 8(1), 1-6. Ona DD, Nevado JB, Cutiongco-de la Paz EC, Ramos JD, Aman AYCL, …, and Sy RG. (2021). Variant rs6596140 of Follistatin-like 4 Gene (FSTL4) May Be Associated with Poor Response to Angiotensin Receptor Blockers (ARBs) among Filipinos. Philippine Journal of Science, Vol. 150 (4): pp. 703-21. Aman AYCL, Sy RG, Nevado JB, Bejarin AP, Reganit PM,…, and Cutiongco-de la Paz EC. (2021). rs17465637 variant of MIA3 may be associated with coronary artery disease among Filipinos. Philippine Journal of Science, Vol. 150 (5): pp. 1051-62. |
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CARDIOVASCULAR DISEASES - INFOGRAPHIC  
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Contact the Microarray Core Laboratory for more information: The UP-NIH Microarray Core Laboratory
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